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Updated: Nov 5, 2025

Isolation, Culture, and Transplantation of Muscle Satellite Cells
Published on: April 8, 2014
An in vitro assay to quantify satellite cell activation using isolated mouse myofibers
Rodrigo Canibano-Fraile1,2,3, Emma Boertjes1,2,3, Stela Bozhilova1,2,3
1Department of Clinical Genetics, Erasmus MC University Medical Center, 3015 GD Rotterdam, the Netherlands.
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Isolated myofibers offer the possibility of in vitro study of satellite cells in their niche. We describe a mouse myofiber isolation assay to assess satellite cell activation by quantifying myofiber-derived satellite cell progeny. The assay allows isolation of myofibers from a mouse using standard equipment and reagents. It can be used to compare satellite cells across different mouse models or to evaluate their response to treatments, offering a valuable complementary tool for in vitro experimentation.

