Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Elemental microanalysis of biological specimens.

G M Roomans1, J Wroblewski, R Wróblewski

  • 1Department of Ultrastructure Research, University of Stockholm, Sweden.

Scanning Microscopy
|June 1, 1988
PubMed
Summary

Complementary elemental microanalysis techniques offer valuable data beyond electron probe X-ray microanalysis for biological specimens. A unified approach to specimen preparation and data analysis is recommended for all microanalytical methods.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A fully capable pianist with a congenital bilateral agenesis of extensor pollicis brevis muscle.

Folia morphologica·2022
Same author

Immunoproteomic analysis of Trichinella spiralis larval crude antigens recognized by sera from patients with trichinellosis after treatment with albendazole.

Tropical biomedicine·2021
Same author

Morphology of sesamoid bones in keyboard musicians.

Folia morphologica·2020
Same author

The use of PIXE in experimental studies of the physiology of human skin epidermis.

Biological trace element research·2013
Same author

Corticosteroids and montelukast: effects on airway epithelial and human umbilical vein endothelial cells.

Lung·2010
Same author

Chloride transport in NCL-SG3 sweat gland cells: channels involved.

Experimental and molecular pathology·2007

Area of Science:

  • Biological sciences
  • Materials science
  • Analytical chemistry

Background:

  • Electron probe X-ray microanalysis is a common technique for elemental microanalysis in biological samples.
  • Limitations exist in electron probe X-ray microanalysis for certain biological specimen analyses.

Purpose of the Study:

  • To explore alternative elemental microanalysis techniques for biological specimens.
  • To identify complementary methods that overcome limitations of electron probe X-ray microanalysis.
  • To propose a unified approach for microanalytical studies in biological sciences.

Main Methods:

  • Review of various elemental microanalysis techniques: electron energy loss spectroscopy, scanning Auger microanalysis, proton, ion, and laser microprobe analysis.
  • Comparison of physical principles and instrumentation of different microanalytical methods.
  • Evaluation of commonalities in specimen preparation, quantitative analysis, and data interpretation.

Main Results:

  • Alternative methods like electron energy loss spectroscopy, scanning Auger microanalysis, and particle/laser probe analyses provide complementary elemental information.
  • These techniques can address limitations inherent in electron probe X-ray microanalysis.
  • Significant commonalities exist across different microanalytical techniques regarding sample handling and data processing.

Conclusions:

  • Elemental microanalysis of biological specimens can benefit from a diverse range of techniques beyond electron probe X-ray microanalysis.
  • A standardized approach to specimen preparation and data analysis is beneficial for all microanalytical methods in biological research.
  • Integrating various microanalytical strategies can enhance the comprehensive understanding of elemental distribution in biological systems.

Related Experiment Videos