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Updated: Nov 5, 2025
Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
Published on: January 7, 2020
FBA: feature barcoding analysis for single cell RNA-Seq
Jialei Duan1, Gary C Hon1,2
1The Green Center, Tecil H. and Ida Green Center for Reproductive Biology Sciences, Dallas, TX, 75390, USA.
Motivation:
Single cell RNA-Seq (scRNA-Seq) has broadened our understanding of cellular heterogeneity and provided valuable insights into cellular functions. Recent experimental strategies extend scRNA-Seq readouts to include additional features, including cell surface proteins and genomic perturbations. These 'feature barcoding' strategies rely on converting molecular and cellular features to unique sequence barcodes, which are then detected with the transcriptome.
Results:
Here, we introduce FBA, a flexible and streamlined package to perform quality control, quantification, demultiplexing, multiplet detection, clustering and visualization of feature barcoding assays.
Availabilityand Implementation:
FBA is available on PyPi at https://pypi.org/project/fba and on GitHub at https://github.com/jlduan/fba.
Supplementary Information:
Supplementary data are available at Bioinformatics online.
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