Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Optimizing Chromatographic Separations01:15

Optimizing Chromatographic Separations

615
Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
615
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

568
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
568
Extraction: Advanced Methods00:56

Extraction: Advanced Methods

714
Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...
714
Methods of Sterilization II: Chemical Methods01:30

Methods of Sterilization II: Chemical Methods

8.2K
In healthcare, the chemical method of sterilization uses chemical sterilants to treat surgical instruments and medical supplies to help prevent the transmission of infectious pathogens to patients. Due to heat sensitivity, most medical supplies and equipment should not be exposed to high temperatures. These parts include rubber, plastic, glass, and other similar elements.
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
8.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Regenerative Endodontics in Teeth With Irreversible Pulpitis: A Scoping Review.

International endodontic journal·2026
Same author

The Use of Silver Fluoride for Oral Health and Well-Being in Aged Care Residents: Protocol for a Cluster Randomized Controlled Trial.

JMIR research protocols·2026
Same author

A novel flowable calcium silicate cement for endodontic therapy in equine teeth.

Equine veterinary journal·2026
Same author

Nonvital Pulp Therapy in Primary Teeth Using Mineral Trioxide Aggregate Obturation: A Retrospective Case Series.

Case reports in dentistry·2026
Same author

Management of Coronal Dens Invaginatus Using Nonsurgical and Surgical Approaches Assisted by a 3D-Printed Model: A Case Report.

Cureus·2025
Same author

A point-of-care testing intervention to improve hepatitis C diagnosis and treatment uptake among people attending Aboriginal community controlled health services: the SCALE-C study.

BMC infectious diseases·2025

Related Experiment Video

Updated: Nov 5, 2025

Automatic Separation and Collection of Cancer-Related Substances from Clinical Samples
08:49

Automatic Separation and Collection of Cancer-Related Substances from Clinical Samples

Published on: January 13, 2023

2.1K

Factors Affecting the Removal Time of Separated Instruments.

Yoshi Terauchi1, Christopher Sexton2, Leif K Bakland3

  • 1Private Endodontic Practice, Tokyo, Japan.

Journal of Endodontics
|May 17, 2021
PubMed
Summary

Retrieving separated endodontic instruments is successful using ultrasonic techniques. Longer instrument fragments and curved root canals significantly increase the time required for removal, impacting endodontic retreatment outcomes.

Keywords:
Canal curvaturedental operating microscopesinstrument retrievalpreparation timeseparated instrumentultrasonic instruments

More Related Videos

Split Point Analysis and Uncertainty Quantification of Thermal-Optical Organic/Elemental Carbon Measurements
10:22

Split Point Analysis and Uncertainty Quantification of Thermal-Optical Organic/Elemental Carbon Measurements

Published on: September 7, 2019

8.5K
Automating Citrus Budwood Processing for Downstream Pathogen Detection Through Instrument Engineering
11:30

Automating Citrus Budwood Processing for Downstream Pathogen Detection Through Instrument Engineering

Published on: April 21, 2023

986

Related Experiment Videos

Last Updated: Nov 5, 2025

Automatic Separation and Collection of Cancer-Related Substances from Clinical Samples
08:49

Automatic Separation and Collection of Cancer-Related Substances from Clinical Samples

Published on: January 13, 2023

2.1K
Split Point Analysis and Uncertainty Quantification of Thermal-Optical Organic/Elemental Carbon Measurements
10:22

Split Point Analysis and Uncertainty Quantification of Thermal-Optical Organic/Elemental Carbon Measurements

Published on: September 7, 2019

8.5K
Automating Citrus Budwood Processing for Downstream Pathogen Detection Through Instrument Engineering
11:30

Automating Citrus Budwood Processing for Downstream Pathogen Detection Through Instrument Engineering

Published on: April 21, 2023

986

Area of Science:

  • Endodontics
  • Dental Materials Science

Background:

  • Separated endodontic instruments can negatively impact treatment success.
  • Ultrasonic techniques combined with dental operating microscopes offer effective removal of separated instruments.

Purpose of the Study:

  • To evaluate the influence of root canal curvature and separated instrument length on the time required for loosening and retrieving instrument fragments.
  • To analyze the efficiency of different retrieval methods.

Main Methods:

  • Retrospective evaluation of 128 separated instrument retrieval procedures.
  • Cone-beam computed tomography (CBCT) for measuring instrument length and canal curvature.
  • Utilized ultrasonic instruments, wire loops, and XP Shapers for fragment removal.
  • Recorded procedure times and performed statistical analysis using log-normal regression, structural equation modeling, and linear regression.

Main Results:

  • All separated instruments were successfully retrieved.
  • 89.8% of instruments were removed using ultrasonic instruments alone (mean time: 221 seconds).
  • Instrument removal time was significantly influenced by both instrument length and root canal curvature.
  • Longer preparation times were observed with increasing separated instrument lengths, particularly when loop devices were necessary.

Conclusions:

  • The preparation phase is crucial for successful separated instrument retrieval.
  • Instrument length and root canal curvature are independent predictors of retrieval time.
  • Increased file lengths and higher degrees of canal curvature generally extend procedure times.