Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Platelet-activating factor binding and metabolism during human platelet aggregation.

F H Valone1

  • 1Department of Medicine, VA Medical Center, San Francisco, CA.

Thrombosis Research
|April 1, 1988
PubMed
Summary

Platelet-activating factor (PAF) binding and metabolism are crucial for platelet activation. Studies show PAF receptor internalization and metabolism are not essential for this process.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bispecific Antibody MDX-210 for Treatment of Advanced Ovarian and Breast Cancer.

Methods in molecular medicine·2011
Same author

Transplantation with low-density autologous PBSC prepared with BDS60 for women with Stage II, III and IV breast cancer.

Cytotherapy·2002
Same author

Dendritic cell-based treatment of cancer: closing in on a cellular therapy.

Cancer journal (Sudbury, Mass.)·2002
Same author

Fluorouracil modulation in colorectal cancer: lack of improvement with N -phosphonoacetyl- l -aspartic acid or oral leucovorin or interferon, but enhanced therapeutic index with weekly 24-hour infusion schedule--an Eastern Cooperative Oncology Group/Cancer and Leukemia Group B Study.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology·2001
Same author

Immunotherapy of hormone-refractory prostate cancer with antigen-loaded dendritic cells.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology·2000
Same author

Priming tissue-specific cellular immunity in a phase I trial of autologous dendritic cells for prostate cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research·2000

Area of Science:

  • Biochemistry
  • Hematology
  • Cell Biology

Background:

  • Platelet-activating factor (PAF) is a potent lipid mediator involved in various physiological and pathological processes.
  • Understanding the kinetics of PAF binding and metabolism is crucial for elucidating platelet activation mechanisms.

Purpose of the Study:

  • To quantify Platelet-activating factor (PAF) binding and metabolism during human platelet aggregation.
  • To determine the role of PAF receptor internalization and metabolism in platelet activation.

Main Methods:

  • Human platelets were stimulated with radiolabeled [3H]PAF to induce aggregation.
  • PAF binding, aggregation, and metabolism were measured over time.
  • Scatchard analysis was used to characterize PAF receptors.

Related Experiment Videos

Main Results:

  • Platelet aggregation and PAF binding peaked rapidly (45-75 sec and 15-60 sec, respectively).
  • Specific PAF receptor binding was reversible at maximal aggregation, with approximately 124 receptors per platelet (Kd = 0.56 nM).
  • Maximal aggregation was induced by ~52 PAF molecules per platelet, with minimal metabolism (<2%) to lysoPAF at peak aggregation.

Conclusions:

  • PAF receptor internalization is not essential for human platelet activation.
  • Significant PAF metabolism is not required for initiating platelet aggregation.