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Hyaluronic Acid Concentration in Female Vocal Folds With Reinke's Edema
Cristiana Vanderlei de Melo Lins1, João Roberto Maciel Martins2, Elsa Yoko Kobayashi2
1Department of Otorhinolaryngology-Head and Neck Surgery, Universidade Federal de São Paulo / Escola Paulista de Medicina, São Paulo, Brazil.
Objective:
The aim of the present study was to investigate hyaluronic acid (HA) concentrations in vocal folds among patients with Reinke's edema.
Study Design:
Prospective and experimental study.
Setting:
Single tertiary center.
Methods:
An HA binding protein isolated from bovine nasal cartilage was used to identify and isolate the HA from samples. Plates coated with biotin-conjugated binding protein and streptavidin-europium conjugate were sequentially incubated with 18 Reinke's edema samples and 11 female vocal fold cover samples from cadavers (the superficial layer of the lamina propria; control group). After the release of europium from streptavidin in enhancement solution, final fluorescence was measured in a fluorometer.
Results:
The mean HA concentration in Reinke's edema vocal folds was significantly higher than that in the control vocal folds (9.2 × 103 vs 0.9 × 103µg/g).
Conclusion:
Vocal fold covers affected by Reinke's edema present a higher concentration of HA than do vocal fold covers with no edema.
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