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Curcumin suppresses renal carcinoma tumorigenesis by regulating circ-FNDC3B/miR-138-5p/IGF2 axis

Li Xue1, Yuhua Tao2, Yanjuan Yuan3

  • 1Departments of Nephrology.

Anti-Cancer Drugs
|May 18, 2021
PubMed

Insights

Curcumin inhibits renal carcinoma by downregulating circ-FNDC3B and upregulating miR-138-5p, impacting the IGF2 pathway. This study reveals curcumin

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Renal carcinoma (kidney cancer) is a significant health concern.
  • The precise mechanisms by which curcumin affects renal carcinoma tumorigenesis are not fully understood.
  • Circular RNAs (circRNAs) and microRNAs (miRNAs) are increasingly recognized for their roles in cancer development.

Purpose of the Study:

  • To elucidate the mechanism of curcumin's action in renal carcinoma.
  • To investigate the roles of circ-FNDC3B, miR-138-5p, and IGF2 in renal carcinoma.
  • To explore the therapeutic potential of curcumin in renal carcinoma.

Main Methods:

  • Quantitative reverse transcription PCR and Western blot to measure molecular levels.
  • Cell proliferation and apoptosis assays (MTT, colony formation, flow cytometry).
  • Dual-luciferase reporter assays to confirm molecular interactions.
  • In vivo xenograft model to assess anti-tumor effects.

Main Results:

  • Elevated circ-FNDC3B and decreased miR-138-5p were observed in renal carcinoma tissues and cells.
  • Curcumin suppressed renal carcinoma cell proliferation and induced apoptosis.
  • Circ-FNDC3B and miR-138-5p modulated curcumin's effects; circ-FNDC3B knockdown promoted apoptosis via miR-138-5p.
  • IGF2 was identified as a target of miR-138-5p and positively regulated by circ-FNDC3B.
  • Curcumin reduced tumor growth in vivo by decreasing circ-FNDC3B.

Conclusions:

  • Curcumin suppresses renal carcinoma tumorigenesis through the circ-FNDC3B/miR-138-5p/IGF2 axis.
  • This pathway offers novel insights into renal carcinoma development and potential therapeutic strategies.
  • Circ-FNDC3B and miR-138-5p are key mediators of curcumin's anti-cancer effects in renal carcinoma.

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