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Related Experiment Video

Updated: Nov 5, 2025

Three-dimensional Tissue Engineered Aligned Astrocyte Networks to Recapitulate Developmental Mechanisms and Facilitate Nervous System Regeneration

Three-dimensional Tissue Engineered Aligned Astrocyte Networks to Recapitulate Developmental Mechanisms and Facilitate Nervous System Regeneration

Published on: January 10, 2018

Neuron and astrocyte aggregation and sorting in three-dimensional neuronal constructs.

Md Fayad Hasan¹, Yevgeny Berdichevsky^2,3

¹Department of Electrical and Computer Engineering, Lehigh University, Bethlehem, PA, USA.

Communications Biology

|May 18, 2021

Summary

This summary is machine-generated.

This study reveals how neurons and astrocytes aggregate and sort in 3D cultures, crucial for engineered neural tissues. Findings show neuron-driven aggregation and astrocyte sheath formation, impacting neural circuit function.

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Published on: November 14, 2016

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Last Updated: Nov 5, 2025

Three-dimensional Tissue Engineered Aligned Astrocyte Networks to Recapitulate Developmental Mechanisms and Facilitate Nervous System Regeneration

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Published on: January 10, 2018

Synaptic Microcircuit Modeling with 3D Cocultures of Astrocytes and Neurons from Human Pluripotent Stem Cells

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The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions

The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions

Published on: November 14, 2016

Area of Science:

Neuroscience
Biomedical Engineering
Computational Biology

Background:

Cell aggregation and sorting are known in non-neuronal 3D cultures.
Limited data exists on collective cell movements in engineered neural tissues.
Cellular rearrangement can impact neural construct morphology and function.

Purpose of the Study:

To investigate neuronal and astrocyte aggregation and sorting in 3D cultures.
To develop a computational model for these cellular dynamics.
To understand how these processes influence engineered neural tissue design.

Main Methods:

Development of linear, adhered 3D neuronal-astrocyte cultures (rat and human).
Creation of an in silico model simulating contraction, clustering, and cell sorting.
Experimental observation and computational analysis of cell behaviors.

Main Results:

Aggregation in 3D cultures is primarily neuron-mediated.
Differential attraction between neurons and astrocytes drives astrocyte sheath formation.
In silico models predicted self-assembled neuronal layers in disk-shaped cultures.
Neuronal activity correlated with local morphological variations.

Conclusions:

Neuron-astrocyte interactions govern aggregation and sorting in 3D neural constructs.
The developed model aids in understanding and designing functional engineered neural tissues.
This research provides insights into the self-organization of neural cells in vitro.