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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Related Experiment Video

Updated: Nov 5, 2025

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
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Nanopore sequencing in non-human forensic genetics.

Rob Ogden1,2, Nina Vasiljevic3, Stefan Prost4,5

  • 1Royal (Dick) School of Veterinary Studies and the Roslin Institute, University of Edinburgh, Edinburgh EH25 9RG, U.K.

Emerging Topics in Life Sciences
|May 18, 2021
PubMed
Summary

Nanopore sequencing offers a cost-effective method for non-human forensic genetics, particularly in wildlife forensics. This technology shows promise for species identification and could become a valuable tool for forensic laboratories.

Keywords:
minionspecies identificationwildlife forensics

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Area of Science:

  • Genetics
  • Forensic Science
  • Bioinformatics

Background:

  • The last decade has seen significant growth in non-human forensic genetics, driven by advances in DNA sequencing.
  • Nanopore sequencing provides a low-cost, high-throughput alternative to traditional sequencing methods.

Purpose of the Study:

  • To review nanopore sequencing technology for non-human forensic applications.
  • To assess its advantages, drawbacks, and potential compared to other next-generation sequencing (NGS) and Sanger sequencing platforms.

Main Methods:

  • Review of current literature on nanopore sequencing in biomonitoring and forensic science.
  • Analysis of nanopore sequencing's performance, error rates, and consensus sequence production.
  • Discussion of validation progress for forensic casework.

Main Results:

  • Nanopore sequencing has demonstrated success in species identification for biomonitoring.
  • Challenges include sequence error rates and standardization of consensus sequences.
  • Recent validation efforts show progress for forensic applications.

Conclusions:

  • Nanopore sequencing holds considerable potential for the future of non-human forensic genetics.
  • It is particularly promising for wildlife forensics and emerging forensic laboratories.