Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Effects of Neuromuscular Electrical Stimulation on Cramp Threshold Frequency and Pain in Adults with Nocturnal Leg Cramps: An EMG-Based Assessment: Randomized Controlled Trial.

Healthcare (Basel, Switzerland)·2026

Same author

Protection of High-Nickel Cathodes from H<sub>2</sub>O-Triggered Electrochemical Degradation Enabled by Moisture-Responsible Dehumidifying Separators.

ACS nano·2026

Same author

Enhanced Polysulfide Redox Reaction of Co/Fe Dual-Atom Catalysts Embedded in Metal-Organic Framework-Derived Microporous Carbon Electrode for Lithium-Sulfur Cells.

Small (Weinheim an der Bergstrasse, Germany)·2026

Same author

Effects of Rhythmic Auditory Stimulation Combined with Real-Time Vibrotactile Feedback on Muscle Activation and Gait in Patients with Stroke: A Randomized Controlled Trial.

NeuroRehabilitation·2026

Same author

Hyperspectral image wavelength band and texture characteristics related to elasticity values for each body part for non-invasive skin elasticity analysis.

Biomedical optics express·2025

Same author

Comparison of Rhythmic Auditory Stimulation Gait Training with and Without Vibrotactile Feedback on Balance and Gait in Persons with Stroke: A Randomized Controlled Trial.

Bioengineering (Basel, Switzerland)·2025

Search research articles

Related Experiment Video

Updated: Nov 5, 2025

Non-Invasive Visualization of Nailbed Microvascular Morphology in Mice Using Capillaroscopy

Non-Invasive Visualization of Nailbed Microvascular Morphology in Mice Using Capillaroscopy

Published on: February 28, 2025

Three-dimensional structure analysis of mouse nails using synchrotron radiation.

HyunJong Yoo¹, YongJu Jung², Sang-Hun Jang³

¹Department of Computer Science and Engineering, Graduate School, Soonchunhyang University, 22, Soonchunhyang-ro, Asan, Chungnam-do 31538, Republic of Korea.

Microscopy (Oxford, England)

|May 18, 2021

Summary

Synchrotron radiation (SR) X-ray imaging offers a non-damaging, high-resolution method for analyzing mouse nail structures. This advanced technique visualizes internal anatomy in 3D, aiding nail disease research and drug development.

Keywords:

hematoxylin and eosin stain nail phase-contrast hard X-ray microscopy semantic segmentation synchrotron radiation three-dimensional imaging

More Related Videos

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Published on: November 5, 2018

Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging

Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging

Published on: March 19, 2019

Related Experiment Videos

Last Updated: Nov 5, 2025

Non-Invasive Visualization of Nailbed Microvascular Morphology in Mice Using Capillaroscopy

Non-Invasive Visualization of Nailbed Microvascular Morphology in Mice Using Capillaroscopy

Published on: February 28, 2025

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Published on: November 5, 2018

Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging

Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging

Published on: March 19, 2019

Area of Science:

Biomedical imaging
Histopathology
Materials science

Background:

Conventional nail disease studies use microscopy and micro-CT, which have limitations.
Microscopy can damage samples and is time-consuming.
Micro-CT lacks sufficient penetration and resolution for microstructures.

Purpose of the Study:

To compare optical microscopy with synchrotron radiation (SR) X-ray imaging for analyzing mouse nail tissue.
To evaluate SR imaging's potential for non-invasive, high-resolution visualization of nail anatomy.

Main Methods:

Comparison of optical microscopy (hematoxylin and eosin staining) and SR X-ray imaging.
Analysis of mouse nail tissue using both techniques.
Three-dimensional (3D) reconstruction and visualization of nail structures.

Main Results:

SR X-ray imaging depicted internal mouse nail structures without physical damage.
SR imaging provided high-resolution, 3D visualization of the nail bed, nail matrix, and hyponychium.
Medically relevant anatomical structures were clearly visualized in 3D.

Conclusions:

SR X-ray imaging is a promising non-invasive tool for analyzing nail tissue.
This technology can advance the study of nail diseases and pharmaceutical research.
SR imaging offers superior visualization of nail microstructures compared to conventional methods.