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Circular RNA circCRIM1 suppresses lung adenocarcinoma cell migration, invasion, EMT, and glycolysis through

S-J Zhang1, J Ma, J-C Wu

  • 1Department of Respiratory, Huaihe Hospital of Henan University, Kaifeng, China.

European Review for Medical and Pharmacological Sciences
|May 18, 2021
PubMed

Insights

This study investigated circular RNA circCRIM1's role in lung adenocarcinoma. It was found to suppress cell migration, invasion, epithelial-mesenchymal transition, and glycolysis by regulating the miR-125b-5p/BTG2 axis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Lung adenocarcinoma (LUAD) is a major cause of cancer-related deaths.
  • Circular RNAs (circRNAs) are emerging as critical regulators in various cancers, including LUAD.
  • The specific role of circCRIM1 in LUAD progression remains largely unexplored.

Purpose of the Study:

  • To investigate the function of circCRIM1 in lung adenocarcinoma.
  • To elucidate the underlying molecular mechanism involving the miR-125b-5p/BTG2 axis.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) to measure gene expression.
  • Cell migration and invasion assays (e.g., Transwell assays).
  • Western blotting to assess protein levels.
  • Glycolysis assays.

Main Results:

  • CircCRIM1 expression was found to be downregulated in LUAD tissues and cell lines.
  • Overexpression of circCRIM1 significantly inhibited LUAD cell migration, invasion, and epithelial-mesenchymal transition (EMT).
  • CircCRIM1 regulated miR-125b-5p, which in turn targeted BTG2, affecting glycolysis.

Conclusions:

  • CircCRIM1 acts as a tumor suppressor in lung adenocarcinoma.
  • The circCRIM1/miR-125b-5p/BTG2 axis represents a potential therapeutic target for LUAD treatment.

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