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Related Experiment Video

Updated: Nov 5, 2025

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells
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Noncontact Multiphysics Probe for Spatiotemporal Resolved Single-Cell Manipulation and Analyses.

Ayoola T Brimmo1,2, Anoop Menachery1, Pavithra Sukumar1

  • 1Division of Engineering, New York University Abu Dhabi (NYUAD), P.O. Box 129188, Abu Dhabi, UAE.

Small (Weinheim an Der Bergstrasse, Germany)
|May 19, 2021
PubMed
Summary
This summary is machine-generated.

A novel noncontact multiphysics probe (NMP) enables precise manipulation of single cells within their native tissue environment. This technology preserves spatiotemporal resolution for advanced cellular network analysis.

Keywords:
biopsyelectropermealizationmicrofluidic probesinge celltissue culturetranscriptomicstransfectiontweezing

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Microfluidics

Background:

  • Cellular heterogeneity and spatial arrangement are crucial for multicellular organisms.
  • Existing single-cell analysis methods often disrupt the physiological environment, losing spatiotemporal data.
  • Understanding cellular networks requires techniques that maintain cells within their native tissue context.

Purpose of the Study:

  • To develop a multifunctional noncontact scanning probe for in-situ single-cell manipulation.
  • To enable precise manipulation of living single cells within their physiological tissue environment.
  • To overcome limitations of current techniques by preserving spatiotemporal resolution.

Main Methods:

  • Development of a noncontact multiphysics probe (NMP) with fluidic apertures and "hump" electrodes.
  • Utilizing electropermeabilization for efficient macromolecule transfer across cell membranes.
  • Demonstrating adjustable spatial manipulation for transfecting adjacent single cells with different DNA vectors.

Main Results:

  • The NMP successfully performed single-cell manipulation, including transfection, within the tissue environment.
  • Demonstrated controllable cytoplasm extraction from living single cells with preserved macromolecule integrity and cell viability.
  • Showcased the NMP's function as an electro-thermal microfluidic whole-cell tweezer.

Conclusions:

  • The NMP provides a powerful multifunctional tool for spatiotemporal single-cell analysis.
  • This technology preserves cellular microenvironments, enabling deeper insights into cellular networks.
  • Offers unprecedented probing capabilities for complex biological systems.