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PATL2 regulated the apoptosis of ovarian granulosa cells in patients with PCOS

Shao-Lan Peng1,2, Qiong-Fang Wu1,3, Qi Xie1,4

  • 1Maternal and Child Health Hospital Affiliated to Nanchang University, Jiangxi Maternal and Child Health Hospital, Nanchang, Jiangxi, P. R. China.

Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology
|May 19, 2021
PubMed
Abstract

Insights

Reduced PATL2 expression in ovarian granulosa cells (GCs) increases apoptosis, contributing to abnormal follicle development in polycystic ovary syndrome (PCOS). This finding highlights PATL2

Area of Science:

  • Reproductive Biology
  • Cellular Biology
  • Endocrinology

Background:

  • Polycystic ovary syndrome (PCOS) is characterized by abnormal follicular development.
  • Increased granulosa cell (GC) apoptosis is a key factor in follicular dysplasia.
  • PATL2 deficiency impairs human oocyte development and is expressed in GCs.

Purpose of the Study:

  • To investigate the function of PATL2 in regulating GC apoptosis.
  • To explore PATL2's role in PCOS-related abnormal follicle development.

Main Methods:

  • Collected GCs from PCOS and normal ovulating women.
  • Utilized the human GC line (KGN) for in vitro studies.
  • Employed flow cytometry, TUNEL staining, immunofluorescence, and Western Blot.

Main Results:

  • PCOS oocytes showed lower maturation and fertilization rates.
  • GCs from PCOS patients exhibited increased apoptosis.
  • Reduced PATL2 expression was observed in PCOS GCs.
  • PATL2 knockdown inhibited GC apoptosis.

Conclusions:

  • Decreased PATL2 levels in ovarian GCs correlate with increased apoptosis.
  • Reduced PATL2 may be linked to the pathogenesis of abnormal follicles in PCOS.
Keywords:
GCsPATL2PCOSapoptosis

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