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Updated: Nov 5, 2025
Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
Published on: May 14, 2020
Erratum: Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
Abstract:
An erratum was issued for: Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis. The author list was updated. The author list was updated from: Eleftheria Saplaoura1, Valentina Perrera2, Friedrich Kragler1 1Max Planck Institute of Molecular Plant Physiology 2Department of Molecular Medicine, Medical School, University of Padua to: Eleftheria Saplaoura1, Valentina Perrera2, Vincent Colot3, Friedrich Kragler1 1Max Planck Institute of Molecular Plant Physiology 2Department of Molecular Medicine, Medical School, University of Padua 3Biologie de l'Ecole Normale Supérieure (IBENS), Paris, France.
Insights
An erratum updates the author list for a study on methylated RNA immunoprecipitation assays in Arabidopsis. This research focuses on m5C modification in plants.
Area of Science:
- Plant Molecular Biology
- Epigenetics
- RNA Biology
Background:
- N6-methyladenosine (m6A) is the most prevalent internal mRNA modification in eukaryotes.
- The 5-methylcytosine (m5C) is a common epitranscriptomic mark in both prokaryotes and eukaryotes.
- m5C modification in RNA has been implicated in various biological processes.
Purpose of the Study:
- To describe a methylated RNA immunoprecipitation (meRIP) assay for studying m5C modification in Arabidopsis.
- To provide a detailed protocol for researchers interested in plant epitranscriptomics.
Main Methods:
- The study details the Methylated RNA Immunoprecipitation Assay (meRIP) protocol.
- Specific steps include RNA extraction, fragmentation, immunoprecipitation using m5C-specific antibodies, and library preparation for sequencing.
Main Results:
- The erratum specifically corrects the author list of the original publication.
- The scientific findings and methods described in the original paper remain unchanged.
Conclusions:
- The updated author list reflects the contributions of all researchers involved in the study.
- The described meRIP assay is a valuable tool for investigating m5C modifications in Arabidopsis RNA.
