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Activation of the cGAS-STING signaling pathway in adenomyosis patients

Yun Lin1, Luying Wang1, Mingzhu Ye1

  • 1Department of Obstetrics and Gynecology, The 3rd Xiangya Hospital of Central South University, Changsha, Hunan, China.

Immunity, Inflammation and Disease
|May 19, 2021
PubMed
Abstract

Insights

The cyclic GMP-AMP synthase-stimulator of interferon genes (cGAS-STING) pathway is activated in adenomyosis, correlating with disease severity and symptoms like dysmenorrhea. This suggests the pathway plays a role in adenomyosis development.

Area of Science:

  • Gynecologic pathology
  • Immunology
  • Molecular biology

Background:

  • Adenomyosis is a condition where endometrial tissue implants within the uterine myometrium.
  • The role of innate immune pathways, such as the cGAS-STING pathway, in adenomyosis pathogenesis is not well understood.

Purpose of the Study:

  • To investigate the activation status of the cGAS-STING pathway in adenomyosis.
  • To explore the correlation between cGAS-STING pathway activation and clinical outcomes in adenomyosis patients.

Main Methods:

  • Quantitative real-time reverse-transcription polymerase chain reaction (qRT-PCR) and immunohistochemistry were used to measure mRNA and protein levels of cGAS-STING pathway components.
  • Tissue samples from adenomyosis patients and controls were analyzed.
  • Correlation analysis was performed between gene expression and clinical parameters like dysmenorrhea and uterine volume.

Main Results:

  • Significantly elevated mRNA and protein levels of cGAS, STING, TANK-binding kinase 1 (TBK-1), interferon-α (IFN-α), IFN-β, and tumor necrosis factor-α (TNF-α) were observed in adenomyosis tissues compared to controls.
  • cGAS and STING gene expression correlated with TBK-1, IFN-β, and TNF-α expression.
  • TBK-1 and TNF-α expression levels were significantly correlated with the severity of dysmenorrhea.

Conclusions:

  • The cGAS-STING pathway is activated in adenomyosis.
  • Pathway activation is linked to clinical manifestations, particularly dysmenorrhea.
  • The cGAS-STING pathway may be a key contributor to the pathogenesis of adenomyosis and a potential therapeutic target.
Keywords:
STINGadenomyosisendometrium cGAS

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