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Enzyme-Linked Immunosorbent Assay

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Related Experiment Video

Updated: Nov 5, 2025

Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques

Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques

Published on: January 12, 2024

Developing a SARS-CoV-2 Antigen Test Using Engineered Affinity Proteins.

Seunghyeon Kim¹, Emma Yee¹, Eric A Miller¹

¹Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Chemrxiv : the Preprint Server for Chemistry

|May 20, 2021

Summary

This summary is machine-generated.

A new method rapidly identified diagnostic tools for COVID-19. This enables fast, large-scale production of paper-based tests for infectious diseases.

Keywords:

affinity proteins cellulose cellulose binding domains cellulose binding modules covid-19 directed evolution enzyme-linked immunosorbent assay flow test strips library screening peptides proteins rapid detection test rcSso7d roll to roll manufacturing thermostable protein yeast surface display

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Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter

Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter

Published on: August 12, 2021

Area of Science:

Biotechnology
Molecular Diagnostics
Infectious Disease Research

Background:

The COVID-19 pandemic highlighted the need for rapid, scalable diagnostic tests.
Current diagnostic technologies face challenges in large-scale manufacturing and accessibility.

Approach:

Utilized the Rapid Affinity Pair Identification via Directed Selection (RAPIDS) method to discover affinity pairs for SARS-CoV-2 nucleocapsid protein (N-protein).
Developed a 10-minute, vertical-flow cellulose paper test integrating the highest sensitivity affinity pair.
Demonstrated compatibility of identified affinity proteins with roll-to-roll printing for mass production.

Key Points:

Discovered multiple affinity pairs for SARS-CoV-2 N-protein within 10 weeks.
Achieved limits of detection at 40 pM and 80 pM in mock swab and saliva samples.
Successfully detected N-protein in clinical swab samples.

Conclusions:

This work enables mass production of cellulose paper-based assays, addressing shortages of current diagnostic materials.
The RAPIDS method and engineered binders show promise for rapid development of diagnostic tests for emerging infectious diseases.