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Updated: Nov 5, 2025

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models
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The inhibitory effect of CTAB on human osteosarcoma through the PI3K/AKT signaling pathway

Wacili Da1, Lin Tao1, Yue Zhu1

  • 1Department of Orthopedics, The First Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China.

International Journal of Oncology
|May 20, 2021
PubMed

Insights

Cetrimonium bromide (CTAB) shows promise as a novel osteosarcoma treatment. This compound effectively inhibits cancer cell proliferation, migration, and invasion while promoting apoptosis, with minimal toxicity observed in vivo.

Area of Science:

  • Oncology
  • Pharmacology
  • Biochemistry

Background:

  • Osteosarcoma (OS) presents significant clinical challenges due to metastasis, recurrence, and multidrug resistance.
  • There is a critical need for novel, effective, and low-toxicity therapeutic agents for osteosarcoma treatment.

Purpose of the Study:

  • To investigate the anti-cancer effects of cetrimonium bromide (CTAB) on osteosarcoma cells.
  • To elucidate the underlying molecular mechanisms of CTAB's action in osteosarcoma.

Main Methods:

  • In vitro studies assessed CTAB's impact on osteosarcoma cell proliferation, cell cycle, migration, and invasion.
  • Caspase-mediated apoptosis and mitochondrial toxicity were evaluated.
  • In vivo studies examined CTAB's efficacy and organ toxicity in a preclinical osteosarcoma model.

Main Results:

  • CTAB inhibited osteosarcoma cell proliferation, migration, and invasion in a dose- and time-dependent manner.
  • CTAB induced G1 phase cell cycle arrest and promoted caspase-mediated apoptosis via the PI3K/AKT pathway.
  • CTAB demonstrated significant anti-tumor activity in vivo without inducing observable organ toxicity, despite noted mitochondrial toxicity in vitro.

Conclusions:

  • Cetrimonium bromide exhibits significant anti-osteosarcoma properties by inhibiting proliferation and metastasis and inducing apoptosis.
  • The PI3K/AKT signaling pathway is implicated in CTAB's mechanism of action.
  • CTAB represents a potential therapeutic candidate for osteosarcoma treatment.
Keywords:
CTABPI3K/AKTapoptosisinvasionmigrationosteosarcomaproliferation

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