Related Experiment Video
Updated: Nov 5, 2025

13:34
Production, Crystallization and Structure Determination of C. difficile PPEP-1 via Microseeding and Zinc-SAD
Published on: December 30, 2016
11.7K
Crystallographic Studies of PDZ Domain-Peptide Interactions of the Scribble Polarity Module
Janesha C Maddumage1,2, Bryce Z Stewart1,2, Patrick O Humbert3,4
1Department of Biochemistry and Genetics, La Trobe University, Bundoora, VIC, Australia.
Methods in Molecular Biology (Clifton, N.J.)
|May 20, 2021
Abstract:
The determination of high-resolution crystal structures of cell polarity regulatory proteins bound to their functional interactors has proven to be invaluable for deciphering the underlying molecular mechanisms. Here we describe methods to identify suitable complexes of cell polarity protein domains bound to interacting ligands with subsequent preparation of such complexes for X-ray crystallographic analysis.

