Functional characterization of VscCD, an important component of the type Ⅲ secretion system of Vibrio harveyi

Vibrio harveyi is a Gram-negative bacterium that occurs widely in the ocean and a kind of pathogenic bacteria associated with vibriosis in grouper. We investigated whether the VscCD protein of the type Ⅲ secretion system (T3SS) was important for pathogenicity of V. harveyi. Mutations to the vscC and vscD genes (ΔvscCD) and complementation of the ΔvscCD mutant (C-ΔvscCD) were created. Moreover, the biological characteristics of the wild-type (WT) and mutant strains of V. harveyi 345 were compared. The results showed that deletion of the vscCD genes had no effect on bacterial growth, swimming/swarming ability, secretion of extracellular protease, or biofilm formation. However, as compared with the V. harveyi 345: pMMB207 (WT⁺) and complementary (C-ΔvscCD) strains, the ΔvscCD: pMMB207 (ΔvscCD⁺) mutant displayed decreased resistance to acid stress, H₂O₂, and antibiotics. In addition, infection of the pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatu) showed that as compared with the WT⁺ and C-ΔvscCD strains, the ΔvscCD⁺ strain significantly reduced cumulative mortality of the host. The colonization ability of the ΔvscCD⁺ mutant in the spleen and liver tissues of the pearl gentian grouper was significantly lower than that of the WT⁺ and C-ΔvscCD strains. In the early stage of infection with the ΔvscCD⁺ strain, the expression levels of IL-1β, IL-16, TLR3, TNF-α, MHC-Iα, and CD8α were up-regulated to varying degrees. As compared with the WT⁺ and C-ΔvscCD strains, luxR expression was significantly up-regulated in the ΔvscCD⁺ strain, while the expression of vcrH and vp1668 was significantly down-regulated. As an important component of the T3SS, VscCD seemed to play a significant role in the pathogenesis of V. harveyi.