Ecotoxicological effect of ketoconazole on the antioxidant system of Daphnia similis

The occurrence of emerging pharmaceutical pollutants (i.e. small drugs, antibiotics) present in aquatic environments shown to be a current environmental problem still without apparent solution. In this regard, the use of ecotoxicological techniques has been shown fundamental for the appraisal of damage to affected living organisms. Herein, ecotoxicological tests were conducted, focusing on the evaluation of the effects of ketoconazole (KTZ) on the antioxidant system of the model body Daphnia similis. In order to study the biochemical changes caused by KTZ in the antioxidant system, the enzymatic biomarkers glutathione S-transferase (GST), catalase (CAT), and ascorbate peroxidase (APX) were monitored. Toxicological tests were conducted using KTZ concentrations (0-10 μg·L^-1). Prolonged exposure to KTZ (336 h) caused changes upon the expression of antioxidant enzymes and simultaneously affected the reproductive system in those organisms. Moreover, a decrease in GST and APX activity was observed caused by KTZ exposure, respectively 79.2% (3.53 μmol min^-1 mg^-1 protein) and 24.4% (0.88 μmol min^-1 mg^-1 protein). On the other hand, it was observed an increase of 27% (0.17 μmol min^-1 mg^-1 protein) in CAT activity. Through this study, it was possible to observe the toxicological effects of KTZ, which proves its action as an oxidative stress-inducing agent and endocrine modifier in daphnids organisms.