Related Experiment Video For Immunofluorescence
Updated: Nov 4, 2025
Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80
Published on: May 3, 2015
Detection of Post-translational Modifications on MYC
Colin J Daniel1, Xiao-Xin Sun1, Yingxiao Chen1
1Department of Molecular and Medical Genetics, Oregon Health and Science University, Portland, OR, USA.
Abstract:
Detection of post-translational modifications in c-Myc is an invaluable tool in assessing Myc status, particularly in cancer. However, it can be challenging to detect these modifications. The evaluation of phosphorylation status of c-Myc can also be challenging with the current commercially available phosphorylation sensitive antibodies. Here, we describe protocols for the immunoprecipitation of endogenous c-Myc to probe for phosphorylation status, as well as the detection of ubiquitination and SUMOylation on c-Myc. We will also discuss the challenges of detecting phosphorylated c-Myc in formalin-fixed paraffin-embedded tissues by immunofluorescence and describe a protocol using a new rat monoclonal antibody we have generated suitable for this purpose.
More Related Videos
07:13Detection of Post-translational Modifications on Native Intact Nucleosomes by ELISA
Published on: April 26, 2011
08:12Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins
Published on: January 8, 2018
Related Concept Videos
02:04Covalently Linked Protein Regulators
These groups modify specific amino acids in a protein....
01:24Tagging and Fusion Proteins