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Comparative study of Legiolert with ISO 11731-1998 standard method-conclusions from a Public Health Laboratory
Javier Checa1, Iago Carbonell1, Neus Manero1
1Public Health Laboratory of L'Hospitalet. Health Service, L'Hospitalet City Council, Cobalt Building. Cobalt street, 57-59, 2nd floor, 08907 L'Hospitalet de Llobregat, Barcelona, Spain.
Legiolert offers a more sensitive and specific method for detecting Legionella pneumophila in water compared to the standard ISO method. This alternative method provides procedural advantages, saving time and resources for laboratories.
Area of Science:
- Microbiology
- Environmental Science
- Public Health
Background:
- Legionella pneumophila causes 96% of Legionnaires' disease and 10% of pneumonia cases.
- Legiolert is a liquid culture method for enumerating L. pneumophila in water.
Purpose of the Study:
- To validate Legiolert as a suitable alternative method for Legionella enumeration in Spanish water samples.
- To compare Legiolert's performance against the standard ISO 11731:1998 method.
Main Methods:
- 118 environmental water samples (potable and non-potable) were analyzed in parallel using Legiolert and the standard method.
- Statistical analysis included ISO 17994:2014 and McNemar's binomial test to compare recovery and detection rates.
Main Results:
- Legiolert showed slightly higher detection rates and concentrations of Legionella compared to the standard method.
- Legiolert demonstrated superior sensitivity and 100% specificity for L. pneumophila detection in potable water.
- Both methods showed equivalent detection for overall Legionella species (L. spp.).
Conclusions:
- Legiolert is a more sensitive and selective alternative to the standard method for L. pneumophila detection.
- The method offers procedural advantages, reducing costs and time for laboratories.
- IDEXX Legiolert is confirmed as a suitable alternative to ISO 11731:1998 for water testing.
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