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Related Experiment Video

Updated: Nov 4, 2025

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization
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Tools for Assessing Cell-Cycle Progression in Plants.

Clara Echevarría1, Crisanto Gutierrez1, Bénédicte Desvoyes1

  • 1Department of Genome Dynamics and Function, Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Nicolás Cabrera 1, Madrid 28049, Spain.

Plant & Cell Physiology
|May 22, 2021
PubMed
Summary
This summary is machine-generated.

Understanding plant cell-cycle dynamics is key for development. This review covers classical and modern methods, including live imaging, for analyzing cell-cycle progression in plants.

Keywords:
ArabidopsisCell cycleLive-imagingMetabolic labelingReporter genesplants

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Area of Science:

  • Plant Biology
  • Developmental Biology
  • Cell Biology

Background:

  • Cell-cycle parameter estimation is vital for understanding organismal development.
  • Various methods exist to assess cell-cycle status in plant tissues.
  • Classical metabolic labeling provides insights into fixed tissues.

Purpose of the Study:

  • To review current tools for plant cell-cycle analysis.
  • To highlight advancements in cell-cycle reporter technologies.
  • To discuss in vivo live imaging for monitoring cell-cycle progression.

Main Methods:

  • Metabolic labeling for fixed tissue analysis.
  • Development of plant cell-cycle reporter fusion proteins.
  • Advanced imaging techniques for in vivo live monitoring.

Main Results:

  • Classical methods remain valuable for fixed tissue studies.
  • Fluorescent protein technology enables novel cell-cycle reporters.
  • In vivo live imaging allows real-time monitoring of plant cell cycles.

Conclusions:

  • A range of tools are available for plant cell-cycle analysis.
  • Technological advancements enhance the study of plant cell proliferation.
  • These methods are crucial for understanding plant development and responses.