Effects of 1,25 Dihydroxyvitamin D₃ on Human Retinal Pigment Epithelial Cell Lines

To assess the effects of 1,25 dihydroxyvitamin D₃ (vitamin D₃) either alone or under oxidative damage on human retinal pigment epithelium cell lines.

The human retinal pigment epithelial cell lines were pretreated with hydrogen peroxide with different concentrations (100-1000 μM) and durations (4, 12 and 24 h) to determine the appropriate dose. A group of cells were treated with vitamin D₃ alone, and another group of cells were co-treated with different concentrations of (10-100 nM) vitamin D₃ and hydrogen peroxide. Anti-cytotoxic, anti-apoptotic and anti-genotoxic effects of vitamin D₃ on the hydrogen peroxide treated cell line were evaluated. In addition, mitochondrial membrane potentials of treated cell lines were measured.

Vitamin D₃ showed statistically significant anti-cytotoxic effects and increased cell viability in all concentrations (p < 0.001). It has also significantly decreased the intracellular ROS generation at concentrations between 10-60 nM and increased intracellular reactive oxygen species in high doses over 90 nM (p < 0.01). When apoptosis was evaluated, vitamin D₃ caused statistically significant decrease in a dose-dependent manner (p < 0.001). In terms of DNA damage which was caused by oxidative stress, it was observed that vitamin D₃ significantly reduced the damage in a dose-dependent manner (p < 0.001). At the doses of 10-50 nM, vitamin D₃ significantly decreased the mitochondrial membrane potential (p < 0.01).

Our study suggests that 1,25 (OH)₂ D3 is capable for alleviating the oxidative damage in ARPE cell lines. With these results, vitamin D is thought to be a therapeutic alternative for the prevention of age-related macular degeneration. This warrants further investigations.