Related Experiment Video
Updated: Nov 4, 2025

Author Spotlight: Advancing Anaerobic Microbiota Research Using a Novel Respirometry Protocol
Published on: April 26, 2024
Potential of anammox process towards high-efficient nitrogen removal in wastewater treatment: Theoretical analysis
Yiyu Li1, Meng Zhang1, Dongdong Xu1
1Department of Environmental Engineering, Zhejiang University, Hangzhou, China.
Abstract:
Anammox process has been widely applied in the wastewater nitrogen removal for its high rate and low cost. However, few researches reported the process potential in treating low-strength nitrogen wastewater. In this study, a sequencing batch reactor (SBR) was taken to explore the feasibility of low-strength nitrogen wastewater treatment by anammox process in theory and practice. The practical operation indicated that the effluent with satisfactory quality (1.90 ± 0.70 mg-TN·L-1) could be achieved, when the SBR was fed with low-strength nitrogen influent (6.20 ± 0.45 mg-NH4+-N·L-1 and 7.96 ± 0.59 mg-NO2--N·L-1). The hydraulic retention time (HRT), nitrogen removal efficiency, nitrogen removal rate (NRR) and hydraulic loading rate of SBR were 5.42 h, 86.5%, 0.054 kg-N·m-3·d-1 and 4.43 m3·m-3·d-1 during the 79-day operation, respectively. The theoretical analysis revealed the potential of anammox SBR. When SBR is stably operated, the maximum NRR would be 0.062 kg-N·m-3·d-1 if the effluent nitrogen was required to be as low as 3 mg·L-1. The NRR value is feasible for engineering. However, considering the lower specific substrates utilization rate in practice, the maximum stable NRR was calibrated and found inefficient afterwards. In order to improve the potential of anammox process, the reactors without back mixing and with periodic bioaugmentation should be taken in priority for the engineering applications. In particular, the bioaugmentation frequency and single addition amount were calculated as 7 d and 0.3 g-VSS·L-1, respectively. The results may provide guidance for the development of high-efficient and stable nitrogen removal process under low-strength condition.
Related Concept Videos
Metabolism of Chemolithotrophs
Environmental Applications of Microorganisms
Overview of Nitrogen Metabolism
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...

