Dual-signaling amplification strategy for glutathione sensing by using single gold nanoelectrodes

A new nanosensor for glutathione (GSH) detection by use of single nanoelectrodes has been developed through a dual-signaling ratiometric amplification strategy. Ag nanoparticles (Ag NPs) metalized DNA1 was modified on an Au nanoelectrode surface. Due to the strong affinity between Ag NP and GSH, Ag NPs could be removed by the addition of GSH. The remaining metalized DNA1 could hardly form a double strand, while the de-metalized DNA1 could hybrid with DNA2 and DNA3 to form a complex structure to adsorb methylene blue (MB), and then the electrochemical signal of differential pulse voltammetry (DPV) from MB oxidation could be observed. With the addition of GSH, the peak current of MB oxidation at about -0.27 V (I_MB) increases, while the signal of Ag oxidation at about 0.1 V (I_Ag) decreases. It was found that there had a linear relationship between the ratio of dual-signal (I_MB/I_Ag) and the GSH concentrations, which could be used to detect GSH. The ratiometric nanosensor is label-free, easy to operate, and can eliminate inherent system errors. Considering the advantages of nanoelectrodes, such as low IR drop, fast response, and small overall dimension, this developed nanosensor can be used for GSH detection living systems (e.g., cell lysate).