Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

11.3K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
11.3K
Transmission Electron Microscopy01:15

Transmission Electron Microscopy

6.3K
In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
6.3K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.6K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.6K
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

12.1K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
12.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Investigation of the chemical structure of core-shell Fe<sub>3</sub>O<sub>4</sub>@Ni<sub>1-<i>x</i></sub> Co <sub><i>x</i></sub> Fe<sub>2</sub>O<sub>4</sub> nanoparticles and its influence on their magnetic properties.

Nanoscale advances·2026
Same author

Highly Transparent Gallium-Doped Zinc Oxide Nanosheets Enabling Stable All-in-One Red-Green-Blue Photodetectors with High Responsivity.

ACS nano·2026
Same author

Transmission Electron Microscopy Analysis of the Microstructure of Nanocrystalline Small-Molecule Semiconductors in Functional Organic Thin-Film Transistors.

ACS applied materials & interfaces·2026
Same author

Tuning the Nonlinear Optical Properties of MoS<sub>2</sub> by Interfacing with Bay- or Imide-Substituted Perylene Diimides.

ACS applied materials & interfaces·2026
Same author

Unraveling the Decomposition Pathways of LaS-TaS<sub>2</sub> Misfit-Layered Compound Nanostructures under Extreme Electrical Currents by In Situ TEM.

The journal of physical chemistry. C, Nanomaterials and interfaces·2025
Same author

Near-Infrared Light-Accelerated Bioorthogonal Drug Uncaging and Photothermal Ablation by Anisotropic Pd@Au Plasmonic Nanorods.

Journal of the American Chemical Society·2025

Related Experiment Video

Updated: Nov 4, 2025

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
14:56

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

3.9K

Comparative image simulations for phase-plate transmission electron microscopy.

Simon Hettler1, Raul Arenal2

  • 1Laboratorio de Microscopías Avanzadas (LMA), Universidad de Zaragoza, Zaragoza, Spain.

Ultramicroscopy
|May 24, 2021
PubMed
Summary
This summary is machine-generated.

This study compares physical phase plates (PPs) for transmission electron microscopy (TEM) imaging. Hole-free phase plates offer superior contrast for biological samples and thin 2D materials.

Keywords:
Image simulationPhase contrastPhase plateSoftwareTransmission electron microscopy

More Related Videos

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
14:09

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

15.9K
Visualization of Organelles In Situ by Cryo-STEM Tomography
08:37

Visualization of Organelles In Situ by Cryo-STEM Tomography

Published on: June 23, 2023

2.6K

Related Experiment Videos

Last Updated: Nov 4, 2025

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
14:56

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

3.9K
Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
14:09

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

15.9K
Visualization of Organelles In Situ by Cryo-STEM Tomography
08:37

Visualization of Organelles In Situ by Cryo-STEM Tomography

Published on: June 23, 2023

2.6K

Area of Science:

  • Materials Science
  • Microscopy
  • Physics

Background:

  • Physical phase plates (PPs) enhance contrast in transmission electron microscopy (TEM).
  • Hole-free or Volta PPs are of recent high interest.
  • Various PP approaches exist for different applications.

Purpose of the Study:

  • To comparatively simulate TEM images using realistic descriptions of various PPs and sample types.
  • To characterize typical image appearance, artifacts, and simulation parameter influences for different PPs.
  • To quantitatively analyze phase-shifting properties and benefits of adjustable PPs.

Main Methods:

  • Comparative TEM image simulations were performed.
  • Realistic descriptions of PPs and diverse sample types were used.
  • Quantitative contrast analysis was conducted.

Main Results:

  • Simulations illustrated typical image appearances and artifacts for different PPs.
  • Hole-free phase plates showed superior phase-shifting for biological applications.
  • Adjustable phase plates offer significant benefits.
  • PPs enhance image interpretability for weak-phase objects like 2D materials.

Conclusions:

  • Hole-free phase plates are highly effective for biological TEM imaging.
  • Adjustable phase plates provide valuable benefits.
  • PPs improve the imaging of atomically thin 2D materials.
  • Free simulation software is available.