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Updated: Nov 4, 2025

Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking
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Structural characterization of PaFkbA: A periplasmic chaperone from Pseudomonas aeruginosa

Qin Huang1, Jing Yang1, Changcheng Li1

  • 1Center of Infectious Diseases, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation Center, Chengdu, China.

Computational and Structural Biotechnology Journal
|May 24, 2021
PubMed

Insights

Pseudomonas aeruginosa

Area of Science:

  • Microbiology
  • Structural Biology
  • Biochemistry

Background:

  • Bacterial Mip-like FK506-binding proteins (FKBPs) possess peptidyl-prolyl-cis/trans-isomerase (PPIase) and chaperone functions.
  • These activities are crucial for various intracellular processes with complex molecular mechanisms.

Purpose of the Study:

  • To elucidate the crystal structure and biochemical functions of the Mip-like protein PaFkbA from Pseudomonas aeruginosa.
  • To investigate the structural basis of PaFkbA's chaperone activity and its interaction with MucD.

Main Methods:

  • X-ray crystallography to determine the structure of PaFkbA.
  • Biochemical assays to characterize PaFkbA's chaperone activity.
  • Site-directed mutagenesis to explore the role of specific domains and regions in PaFkbA function.

Main Results:

  • The crystal structure of PaFkbA revealed key features, including a hinge region in the connecting helix crucial for conformational transitions.
  • PaFkbA demonstrated chaperone activity for the periplasmic protein MucD, a regulator of alginate biosynthesis.
  • Inter-domain motions, involving N-terminal dimerization, are essential for PaFkbA's chaperone function.

Conclusions:

  • Structural and biochemical data provide insights into the mechanism of FKBP chaperone activity.
  • PaFkbA plays a role in regulating alginate biosynthesis in Pseudomonas aeruginosa through its interaction with MucD.
Keywords:
Chaperone activityCrystal structureFKBP-type proteinInter-domain rearrangementPseudomonas aeruginosa

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