Mini review: Enzyme-based DNA synthesis and selective retrieval for data storage.

Area of Science:

• Biotechnology
• Data Storage
• Molecular Biology

Background:

• Digital data storage needs are rapidly increasing.
• DNA synthesis offers a high-density data storage solution.
• Current DNA data storage relies on chemical synthesis.

Purpose of the Study:

• To review enzymatic methods for DNA data storage.
• To highlight advancements in enzyme-based DNA synthesis.
• To discuss enzyme-based DNA retrieval techniques.

Main Methods:

• Summarizing diverse enzymatic approaches for DNA synthesis.
• Reviewing enzyme-based methods for DNA amplification and denaturation.
• Examining the use of terminal deoxynucleotidyl transferase in DNA data storage.

Main Results:

• Enzymatic DNA synthesis presents a novel alternative to chemical methods.
• Enzymatic techniques facilitate efficient synthesis of single-stranded DNA (ssDNA).
• Enzyme-based amplification and denaturation enable selective data retrieval.

Conclusions:

• Enzymatic methods show significant promise for DNA data storage.
• These methods can improve the efficiency of writing and reading DNA data.
• Further development of enzymatic approaches could revolutionize data archiving.