Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

12.2K
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
12.2K
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

792
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
792
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

498
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
498
Atomic Force Microscopy01:08

Atomic Force Microscopy

3.8K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
3.8K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

11.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
11.3K
Microbial Morphologies01:29

Microbial Morphologies

1.4K
Bacterial and archaeal cells exhibit remarkable diversity in shape and structure, critical in their adaptability and functionality. Among bacteria, the most commonly observed shapes include cocci and bacilli. Cocci are spherical and may exist singly or in groupings such as pairs (diplococci), chains (streptococci), clusters (staphylococci), or tetrads. Bacilli, in contrast, are rod-shaped and can also occur as single cells, in pairs, or chains, depending on their environmental and genetic...
1.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Uncovering the mechanisms of clinically relevant altered antibiotic responses of <i>Staphylococcus aureus</i> under wound infection-mimetic conditions.

iScience·2026
Same author

Epigenetic control of telomeric RNA maintains heterochromatin in telomerase-driven cancers.

Signal transduction and targeted therapy·2026
Same author

Cell Cycle Phases, Their Effect on Cell Mechanical Properties, and the Impact on <i>Candida</i>-Host-Cell Interactions.

ACS applied materials & interfaces·2025
Same author

Identification of targetable vulnerabilities of PLK1-overexpressing cancers by synthetic dosage lethality.

Cell genomics·2025
Same author

A novel role for Neurog2 in MYCN driven neuroendocrine plasticity of prostate cancer.

Oncogene·2025
Same author

Blocking the shikimate pathway amplifies the impact of carvacrol on biofilm formation in <i>Candida albicans</i>.

Microbiology spectrum·2025
Same journal

Correction: Metagenomic and ribosomal transcript profiles of diabetic foot osteomyelitis in Hispanic patients: underestimated bacteria in biofilm persistence.

Frontiers in cellular and infection microbiology·2026
Same journal

Correction: Antigen-specific Th1 cytokine markers and protection against tuberculosis: a systematic review and meta-analysis stratified by progression to active disease and sustained IGRA conversion.

Frontiers in cellular and infection microbiology·2026
Same journal

Microbial dysbiosis and inferred functional profiling reveals the potential role of <i>Methylobacterium</i> in prostate cancer.

Frontiers in cellular and infection microbiology·2026
Same journal

High-risk lineages shape the resistome and virulome of multidrug-resistant <i>Pseudomonas aeruginosa</i>.

Frontiers in cellular and infection microbiology·2026
Same journal

Prevalence and detection of <i>Brucella</i> infection in people with fever of unknown origin in Inner Mongolia, China.

Frontiers in cellular and infection microbiology·2026
Same journal

Editorial: Exploring the interface of mucosal vaccines and immune system modulation.

Frontiers in cellular and infection microbiology·2026
See all related articles

Related Experiment Video

Updated: Nov 4, 2025

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
13:43

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Published on: June 24, 2013

14.3K

AFM-Based Correlative Microscopy Illuminates Human Pathogens.

Supriya V Bhat1, Jared D W Price1, Tanya E S Dahms1

  • 1Department of Chemistry and Biochemistry, University of Regina, Regina, SK, Canada.

Frontiers in Cellular and Infection Microbiology
|May 24, 2021
PubMed
Summary
This summary is machine-generated.

Atomic force microscopy (AFM) combined with optical microscopy offers new insights into microbial pathogenicity. This correlative approach reveals host-pathogen interactions and physical properties crucial for understanding disease.

Keywords:
atomic force microscopy (AFM)bacteriacorrelative microspectroscopy and microscopyfungihost-pathogen interactionmicrobespathogenicityviruses

More Related Videos

Contact Mode Atomic Force Microscopy as a Rapid Technique for Morphological Observation and Bacterial Cell Damage Analysis
05:34

Contact Mode Atomic Force Microscopy as a Rapid Technique for Morphological Observation and Bacterial Cell Damage Analysis

Published on: June 30, 2023

1.9K
Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry
08:51

Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry

Published on: September 15, 2020

4.3K

Related Experiment Videos

Last Updated: Nov 4, 2025

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
13:43

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Published on: June 24, 2013

14.3K
Contact Mode Atomic Force Microscopy as a Rapid Technique for Morphological Observation and Bacterial Cell Damage Analysis
05:34

Contact Mode Atomic Force Microscopy as a Rapid Technique for Morphological Observation and Bacterial Cell Damage Analysis

Published on: June 30, 2023

1.9K
Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry
08:51

Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry

Published on: September 15, 2020

4.3K

Area of Science:

  • Microbiology
  • Biophysics
  • Pathogenesis Research

Background:

  • Microbial virulence factors are key to pathogenicity, but understanding disease transmission, antimicrobial resistance, and host factors remains challenging.
  • Current knowledge gaps exist in directly observing host-pathogen interactions and confirming pathogenicity phenotypes.

Purpose of the Study:

  • To review recent advances in understanding pathogens and their interactions with hosts.
  • To highlight the application of atomic force microscopy (AFM) in studying pathogenicity.
  • To explore the potential of correlative AFM-optical microscopy for host-pathogen interaction studies.

Main Methods:

  • Utilizing atomic force microscopy (AFM) for high-resolution surface topography and mechanical property quantification (pN scale).
  • Combining AFM with various optical microscopy techniques for simultaneous surface and internal cellular analysis.
  • Employing correlative AFM-optical microspectroscopy and microscopy to investigate host-pathogen interfaces.

Main Results:

  • AFM provides detailed insights into pathogen surface properties and physical interactions with host cells.
  • Correlative microscopy visualizes both external pathogen-host interactions and internal cellular responses.
  • This integrated approach enhances the understanding of molecular signaling at host-pathogen interfaces.

Conclusions:

  • Correlative AFM-optical microscopy is a powerful tool for dissecting complex host-pathogen interactions.
  • These advanced imaging techniques are crucial for addressing current challenges in pathogenicity research.
  • Further development and application of these methods will deepen our understanding of microbial diseases.