Related Experiment Video

Updated: Nov 4, 2025

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
08:56

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

11.1K

Role of N6-Methyladenosine RNA Modification in Cardiovascular Disease

Dandan Song1,2, Jianhua Hou3, Junduo Wu4

  • 1Department of Clinical Laboratory, Second Hospital of Jilin University, Changchun, China.

Frontiers in Cardiovascular Medicine
|May 24, 2021
PubMed

Insights

Cardiovascular disease (CVD) involves RNA modifications like N6-methyladenosine (m6A). This review explores m6A in coding and non-coding RNAs, highlighting their roles in CVD development and potential therapeutic targets.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cardiology

Background:

  • Cardiovascular disease (CVD) remains a primary global cause of death and disability.
  • N6-methyladenosine (m6A) is a crucial RNA modification implicated in CVD pathogenesis.
  • While m6A in coding RNAs is studied, its role in non-coding RNAs (ncRNAs) in CVD is less understood.

Purpose of the Study:

  • To review current knowledge on m6A modifications in both coding and non-coding RNAs.
  • To elucidate the regulatory roles of m6A-modified RNAs in the development of CVD.
  • To identify potential avenues for future research and therapeutic strategies targeting m6A pathways in CVD.

Main Methods:

  • Comprehensive literature review of studies on m6A modifications in CVD.
  • Analysis of research focusing on m6A in coding RNAs and its association with cardiovascular conditions.
  • Synthesis of findings related to m6A modifications in ncRNAs and their impact on CVD.

Main Results:

  • m6A modifications in coding RNAs significantly contribute to CVD development.
  • Emerging evidence suggests m6A also regulates ncRNAs, impacting CVD.
  • The interplay between m6A, coding RNAs, and ncRNAs offers new insights into CVD mechanisms.

Conclusions:

  • m6A modifications are critical regulators in CVD, affecting both coding and non-coding RNAs.
  • Further investigation into m6A-modified ncRNAs is essential for a comprehensive understanding of CVD.
  • Targeting m6A pathways presents a promising strategy for CVD treatment.

Keywords:
CVDM6AmRNAsmodificationnon-coding RNAs

More Related Videos

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry08:45

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Published on: April 21, 2022

2.6K
Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis08:50

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

6.9K

Related Concept Videos

RNA Editing02:23

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.3K
RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
34.4K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
8.1K
Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
12.2K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
7.6K
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
6.0K