Protocol for mouse adult neural stem cell isolation and culture
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Updated: Sep 17, 2025
Isolation and Culture of Embryonic Mouse Neural Stem Cells
Published on: November 11, 2018
Ahmed K M A Ahmed1,2, Toke Jost Isaksen1, Toshihide Yamashita1,2,3,4
1Department of Molecular Neuroscience, Graduate School of Medicine, Osaka University, 2-2, Yamadaoka, Suita, Osaka 565-0871, Japan.
Abstract:
This protocol entails a simple method for isolation, culturing, and in vitro differentiation of adult neural stem cells from the dentate gyrus in the hippocampus and the subventricular zone of adult mice. Cultured adult neural stem cells are an important in vitro model to investigate stem cell properties such as proliferation and differentiation and to expand the understanding of plasticity in the adult brain. For complete details on the use and execution of this protocol, please refer to Isaksen et al. (2020).
