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DNM3OS Facilitates Ovarian Cancer Progression by Regulating miR-193a-3p/MAP3K3 Axis

Lei He1, Guolin He2

  • 1Department of Gynecology and Obstetrics, Key Laboratory of Obstetrics and Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education, West China Second Hospital, Sichuan University, Chengdu, China.

Yonsei Medical Journal
|May 24, 2021
PubMed
Abstract

Insights

Long non-coding RNA DNM3OS promotes ovarian cancer (OC) progression by enhancing cell proliferation, migration, and invasion. This oncogenic lncRNA functions through an miR-193a-3p/MAP3K3 signaling axis, indicating a potential therapeutic target for OC.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) play crucial roles in cancer development.
  • The specific function of lncRNA DNM3 opposite strand/antisense RNA (DNM3OS) in ovarian cancer (OC) is not well understood.

Purpose of the Study:

  • To investigate the biological roles of DNM3OS in OC.
  • To elucidate the underlying molecular mechanisms of DNM3OS in OC progression.

Main Methods:

  • Quantitative real-time PCR and Western blot to assess gene and protein expression.
  • Cell proliferation, migration, and invasion assays (CCK-8, EdU, Transwell).
  • Luciferase reporter and RNA immunoprecipitation assays to confirm molecular interactions.

Main Results:

  • DNM3OS expression is upregulated in OC tissues and associated with poor prognosis.
  • DNM3OS overexpression promotes OC cell proliferation, migration, and invasion, and epithelial-mesenchymal transition (EMT).
  • DNM3OS directly interacts with miR-193a-3p, which targets MAP3K3, forming an miR-193a-3p/MAP3K3 axis.

Conclusions:

  • DNM3OS acts as an oncogenic lncRNA in ovarian cancer.
  • DNM3OS promotes OC malignancy by regulating the miR-193a-3p/MAP3K3 axis.
  • DNM3OS represents a potential therapeutic target for ovarian cancer treatment.
Keywords:
DNM3OSMAP3K3lncRNAmiR-193a-3povarian cancer

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