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The SWI/SNF complex in eosinophilic and non eosinophilic chronic rhinosinusitis
Katarzyna Kowalik1, Martyna Waniewska-Leczycka1, Elzbieta Sarnowska2
1Department of Otorhinolaryngology, Faculty of Medicine and Dentistry, Medical University of Warsaw, Warsaw, Poland.
Summary
The SWI/SNF complex was found in both eosinophilic (eCRS) and non-eosinophilic (neCRS) chronic rhinosinusitis, but at lower levels in eCRS. Its expression negatively correlated with eosinophil counts and clinical symptoms in eCRS.
Area of Science:
- Immunology
- Pathophysiology
- Molecular Biology
Background:
- Chronic rhinosinusitis (CRS) is categorized into eosinophilic (eCRS) and non-eosinophilic (neCRS) subtypes based on tissue infiltration.
- The SWI/SNF chromatin remodeling complex is implicated in various cellular processes and may play a role in CRS pathogenesis.
Purpose of the Study:
- To investigate the expression levels of SWI/SNF complex subunits in eCRS and neCRS.
- To determine the correlation between SWI/SNF expression, blood eosinophil count (BEC), and histopathology eosinophil count (HPEC) in both CRS subtypes.
Main Methods:
- Immunohistochemical analysis of sinonasal mucosa from 96 CRS patients (68 eCRS, 28 neCRS) to assess SWI/SNF protein expression.
- Quantification of eosinophils in tissue (HPEC) and blood (BEC) to characterize CRS subtypes and correlate with SWI/SNF levels.
- Evaluation of clinical findings using Lund-Mackay (L-M) and Sino-Nasal Outcome Test-22 (SNOT-22) scores.
Main Results:
- SWI/SNF complex subunits were detected in both eCRS and neCRS tissues, with significantly lower expression observed in eCRS.
- A negative correlation was found between SWI/SNF expression and both HPEC and BEC across all CRS patients.
- BEC and HPEC positively correlated with clinical symptom scores (L-M, SNOT-22), whereas SWI/SNF expression showed a negative correlation with these clinical parameters.
Conclusions:
- The SWI/SNF complex is present in both eCRS and neCRS, but its expression is reduced in eCRS.
- The observed negative correlation between SWI/SNF expression and eosinophil counts/clinical severity in eCRS warrants further investigation to elucidate its functional significance.
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