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Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
Published on: June 3, 2018
A gold-based strip sensor for the detection of benzo[a]pyrene in edible oils
Jingjing Yao1, Xinxin Xu1, Liqiang Liu1
1State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China. murel@163.com xcl@jiangnan.edu.cn and International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.
Abstract:
This report describes the development of a sensitive and broadly specific indirect competitive enzyme-linked immunosorbent assay (icELISA) and a gold nanoparticle-based immunochromatographic strip (GNP-ICS) assay for the detection of benzo[a]pyrene (B[a]P), using an anti-B[a]P monoclonal antibody (mAb). A broad-specific anti-B[a]P mAb (4E8) was raised from two types of haptens, with half maximal inhibitory concentrations and limits of detection (LOD) values of 2.51 and 0.54 ng mL-1, respectively. In addition, the cross-reactivity was up to 390% with structurally related compounds. The GNP-ICS assay based on a GNP-labeled mAb showed broad specificity in the detection of B[a]P and its analogues, with cut-off and visual LOD values of 100 and 10 ng mL-1, respectively. Furthermore, the recoveries from the developed icELISA and GNP-ICS assay in edible oil samples spiked with B[a]P were validated by high-performance liquid chromatography-fluorescence detection. The results revealed that the icELISA could reliably detect B[a]P in edible oils.
