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Updated: Nov 4, 2025

Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

Published on: October 10, 2013

Fiber bundle-based chemiluminescence array detection.

Gen Chen¹, Tan He, Song Sun

¹National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, Anhui 230026, China. cgao@ustc.edu.cn.

Analytical Methods : Advancing Methods and Applications

|May 24, 2021

Summary

This summary is machine-generated.

A new fiber bundle system enhances chemiluminescence detection for multiple samples. This technology offers 50x greater efficiency and avoids vignetting, improving clinical diagnostics and bioassays.

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Area of Science:

Analytical Chemistry
Biomedical Engineering
Optical Physics

Background:

Chemiluminescence (CL) is crucial for clinical diagnostics.
Multiplex analysis requires sensitive, simultaneous detection of multiple CL samples.
Existing lens-based systems face trade-offs between aperture and field of view.

Purpose of the Study:

To develop a fiber bundle-based system for efficient, simultaneous detection of multiple chemiluminescent samples.
To overcome limitations of lens-based systems in multiplex chemiluminescence analysis.

Main Methods:

A fiber bundle directly couples to a charge-coupled device (CCD).
The other end of the fiber bundle is divided into sub-bundles aligned above a chemiluminescence sample array.
Optical fibers' large numerical aperture and high transmittance are utilized.

Main Results:

The fiber bundle system achieved approximately 50 times higher chemiluminescence collection efficiency compared to lens-based systems.
The system demonstrated no vignetting, unlike lens-based imaging systems with large fields of view.
Simultaneous and efficient detection of multiple chemiluminescent samples was achieved.

Conclusions:

The fiber bundle-based system offers a promising advancement for multiplex chemiluminescence detection.
This technology can improve sensitivity and efficiency in clinical diagnostics and bioassays.
The system overcomes key limitations of conventional lens-based approaches.