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Related Experiment Video

Updated: Nov 4, 2025

Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments

Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments

Published on: December 21, 2017

Choosing Fluorescent Probes and Labeling Systems.

Kimberly Jacoby-Morris¹, George H Patterson²

¹Section on Biophotonics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.

Methods in Molecular Biology (Clifton, N.J.)

|May 24, 2021

Summary

This summary is machine-generated.

Choosing the right fluorescent probe and labeling method is crucial for successful fluorescence microscopy experiments in living cells. This overview discusses various probe types and labeling techniques to guide researchers in selecting optimal systems for their assays.

Keywords:

Fluorescent probes Fluorescent proteins Fluorescent tags Fluorophores Labeling

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Area of Science:

Cell biology
Biochemistry
Microscopy

Background:

Fluorescence microscopy is vital for studying biological processes in living cells.
Selecting appropriate fluorescent probes and labeling methods is critical for experimental success.

Purpose of the Study:

To provide an overview of fluorescent probe types and labeling methods.
To guide researchers in selecting optimal fluorophores and labeling systems for fluorescence-based assays.

Main Methods:

Discussion of advantages and disadvantages of different fluorescent probe types.
Evaluation of various protein labeling methods.
Outline of relevant protocols.

Main Results:

Comparison of different fluorophores and labeling strategies.
Identification of key considerations for probe and labeling system selection.

Conclusions:

Informed selection of fluorescent probes and labeling techniques enhances the efficacy of fluorescence microscopy.
Proper methodology is essential for accurate investigation of cellular mechanisms.