Related Experiment Video
Updated: Nov 4, 2025

Imaging G-protein Coupled Receptor GPCR-mediated Signaling Events that Control Chemotaxis of Dictyostelium Discoideum
Published on: September 20, 2011
Imaging GPCR-Mediated Signal Events Leading to Chemotaxis and Phagocytosis
1Chemotaxis Signal Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Disease, NIH, Rockville, MD, USA.
Abstract:
Eukaryotic phagocytes locate microorganisms via chemotaxis and consume them through phagocytosis. The social amoeba Dictyostelium discoideum is a stereotypical phagocyte and a well-established model to study both processes. Recent studies show that a G-protein-coupled receptor (fAR1) mediate a signaling network to control reorganization of the actin cytoskeleton leading both the directional cell movement and the engulfment of bacteria. Many live cell imaging methods have been developed and applied to monitor these signaling events. In this chapter, we will introduce how to measure GPCR-mediated signaling events for cell migration and phagocytosis in Dictyostelium.
More Related Videos
08:24Imaging G Protein-coupled Receptor-mediated Chemotaxis and its Signaling Events in Neutrophil-like HL60 Cells
Published on: September 14, 2016
07:41A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators
Published on: February 20, 2018
Related Concept Videos
Chemotaxis and Direction of Cell Migration
IP3/DAG Signaling Pathway
G Protein-coupled Receptors
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
GPCRs Regulate Adenylyl Cylase Activity
G-protein Coupled Receptors
Amplifying Signals via Second Messengers