Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

What is Genetic Engineering?00:49

What is Genetic Engineering?

76.5K
Overview
76.5K
CRISPR01:59

CRISPR

54.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
54.0K
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

15.5K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
15.5K
Gene Therapy00:59

Gene Therapy

26.3K
Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be...
26.3K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

788
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
788
Homologous Recombination02:31

Homologous Recombination

57.9K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
57.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Human Germline Genome Editing, 10 Years On.

JAMA·2025
Same author

Germline Genome Editing May Never Have Any Clinical Utility.

The American journal of bioethics : AJOB·2025
Same author

Whose Genome? Which Genetics?

The American journal of bioethics : AJOB·2023
Same author

Global health and global governance of emerging biomedical technologies.

Journal of medical ethics·2023
Same author

Trojan Horses, Clinical Utility, and Parfitian Puzzles.

The American journal of bioethics : AJOB·2022
Same author

Reactions to the National Academies/Royal Society Report on <i>Heritable Human Genome Editing</i>.

The CRISPR journal·2020

Related Experiment Video

Updated: Nov 4, 2025

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

34.7K

Gene Editing: How Can You Ask "Whether" If You Don't Know "How"?

Bryan Cwik

    The Hastings Center Report
    |May 24, 2021
    PubMed
    Summary

    Ethical discussions on gene editing are shifting. Research now focuses on *how* to perform gene editing ethically, which is a prerequisite for debating *whether* it should be done.

    Area of Science:

    • Bioethics
    • Gene Editing Technologies
    • Translational Science

    Background:

    • Ethical discourse on gene editing has predominantly focused on permissibility and desirability.
    • Existing research on responsible translational pathways for human germline gene editing has faced criticism for addressing secondary concerns.

    Purpose of the Study:

    • To highlight the primacy of 'how' questions in ethical gene editing discussions.
    • To argue that understanding the ethical implementation of gene editing precedes debates on its overall permissibility.

    Main Methods:

    • Literature review and conceptual analysis of ethical frameworks for gene editing.
    • Examination of the relationship between ethical implementation models and permissibility debates.

    Main Results:

    Keywords:
    emerging medical technologiesgene editingreproductive medicineresearch ethicstranslational research

    More Related Videos

    A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
    10:07

    A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

    Published on: August 25, 2017

    8.1K
    Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
    09:43

    Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

    Published on: January 3, 2025

    2.8K

    Related Experiment Videos

    Last Updated: Nov 4, 2025

    Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
    09:51

    Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

    Published on: May 25, 2018

    34.7K
    A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
    10:07

    A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

    Published on: August 25, 2017

    8.1K
    Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
    09:43

    Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

    Published on: January 3, 2025

    2.8K
    • The ethical 'how' of gene editing is a foundational question, logically preceding 'whether' questions.
    • Developing models for responsible clinical use is essential before assessing desirability and permissibility.

    Conclusions:

    • Ethical considerations for gene editing necessitate a focus on practical implementation pathways.
    • Prioritizing 'how' questions will facilitate more productive ethical debates on gene editing applications.