Using saturated absorption for superresolution laser scanning transmission microscopy
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Updated: Nov 4, 2025

Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks
Published on: January 3, 2017
Kentaro Nishida1,2, Hikaru Sato2, Ryosuke Oketani2
1AIST-Osaka University Advanced Photonics and Biosensing Open Innovation Laboratory, AIST, Suita, Osaka, Japan.
Abstract:
We improved the three-dimensional spatial resolution of laser scanning transmission microscopy by exploiting the saturated absorption of dye molecules. The saturated absorption is induced by the high-intensity light irradiation and localises the signal within the centre of the focal spot. Our numerical calculation indicates that the spatial resolution in transmission imaging is significantly improved for both lateral and axial directions using nonlinear transmitted signals induced by saturated absorption. We experimentally demonstrated the improvement of the three-dimensional resolution by observing fine structures of stained rat kidney tissues, which were not able to be visualised by conventional laser scanning transmission microscopy.
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