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Related Concept Videos

Microbial Growth Measurement: Indirect Methods01:27

Microbial Growth Measurement: Indirect Methods

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Estimating microbial growth is essential for understanding population dynamics and environmental adaptations. Indirect methods provide valuable insights by measuring parameters such as turbidity, metabolic activity, and biomass, enabling efficient and reproducible assessments.During exponential growth, microbial cells scatter light proportionally to their biomass, a principle used in turbidity measurements. About one million cells per milliliter produce detectable scattering, which a...
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Direct methods for measuring microbial populations in a culture are essential tools in microbiology, providing quantitative data for various applications. Among these, microscopic counts, plate counts, and serial dilution are widely used techniques, each with unique principles and applications.Microscopic CountsMicroscopic counting involves the use of a Petroff-Hausser chamber, a specialized microscope slide with a grid and defined depth. By observing a liquid culture under a microscope,...
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Related Experiment Video

Updated: Nov 4, 2025

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture
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Refractive Index Sensing for Measuring Single Cell Growth.

Arif E Cetin1, Seda Nur Topkaya2, Ozden Yalcin-Ozuysal3

  • 1Izmir Biomedicine and Genome Center, Balcova, Izmir 35340, Turkey.

ACS Nano
|May 24, 2021
PubMed
Summary

A novel plasmonic assay platform precisely measures adherent cell weight and growth in real-time. This technology reveals cell growth heterogeneity and assesses drug responses, offering broad applications in research and clinical settings.

Keywords:
cell-growth profilingcellular metabolismfunctional assayslabel-free biosensingnanohole arraysplasmonics

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Area of Science:

  • Biophysics
  • Cell Biology
  • Assay Development

Background:

  • Assessing cell growth on substrates is crucial for understanding cell biophysics and drug responses.
  • Existing methods like optical techniques and microfluidics have limitations in sensitivity, reliability, and sample requirements.

Purpose of the Study:

  • To introduce a plasmonic functional assay platform for precise, real-time measurement of adherent cell weight and growth dynamics.
  • To demonstrate the platform's capability in assessing cell growth heterogeneity and evaluating therapeutic responses.

Main Methods:

  • Development and application of a plasmonic assay platform to measure individual adherent cell weight.
  • Real-time monitoring of cell growth kinetics over short intervals (10 min).
  • Investigation of MCF-7 cell growth under varying conditions (serum starvation, low osmolarity) and response to treatments (putrescine, DFMO).

Main Results:

  • The platform accurately determined individual cell growth rates and population growth profiles, revealing heterogeneity.
  • It successfully differentiated growth profiles of MCF-7 cells and ODC-overproducing variants, and showed putrescine's rescue effect.
  • The assay demonstrated differential responses of MCF-7 cells to difluoromethylornithine (DFMO), distinguishing sensitive and resistant subpopulations.

Conclusions:

  • The plasmonic platform offers a sensitive and rapid method for measuring adherent cell growth and intracellular activities.
  • It can assess cell population heterogeneity and predict therapeutic responses to drugs like DFMO.
  • This technology holds significant potential for basic research and clinical applications in cell biology and oncology.