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Updated: Nov 4, 2025

Author Spotlight: Technologies and Challenges in Elemental Analysis of Food Samples
Published on: December 22, 2023
An optimized method for sample preparation and elemental analysis of extra-virgin olive oil by inductively coupled
Maria Luisa Astolfi1, Elisabetta Marconi2, Giulia Vitiello1
1Department of Chemistry, Sapienza University, Piazzale Aldo Moro 5, 00185 Rome, Italy.
Abstract:
The accurate determination of trace elements in vegetable edible oils is still an analytical challenge, owing to their low concentration levels and the complex matrix of the vegetable oils. The aim of this study was to develop a fast and simple analytical method to quantify 45 elements in small mass samples (0.5 g) of extra virgin olive oils by inductively coupled plasma mass spectrometry. To evaluate the best and fastest sample preparation procedure, ultrasonic extraction and wet digestion methods were compared using oil certified reference material with different reagent mixtures, reagent volumes, and times for sample extraction or digestion. The use of 5 mL reagent mixture F [10% (v/v) HNO3 and H2O2, 2:1 (v/v)] for sample digestion in a water bath (95 °C, 40 min) was found to produce satisfactory results in all cases as validated from sample recovery experiments over three different extra virgin olive oil samples.
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