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Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
Published on: June 3, 2018
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Development of an advanced DNA biosensor for pathogenic Vibrio cholerae detection in real sample
M R Ali1, M S Bacchu1, M A A Setu2
1Dept. of Chemical Engineering, Jashore University of Science and Technology, Jashore, 7408, Bangladesh; Laboratory of Nano-bio and Advanced Materials Engineering (NAME), Jashore University of Science and Technology, Jashore, 7408, Bangladesh.
Biosensors & Bioelectronics
|May 24, 2021
Summary
A novel electrochemical biosensor utilizing gold nanocubes detects Vibrio cholerae DNA with high sensitivity and selectivity. This DNA biosensor offers rapid pathogen screening for clinical diagnostics, food safety, and environmental monitoring.
Area of Science:
- * Nanomaterials Science
- * Electrochemistry
- * Biosensor Technology
Background:
- * Rising global microbial diseases necessitate rapid and accurate pathogen detection.
- * Existing methods for pathogenic bacteria quantification face limitations in speed and sensitivity.
- * Vibrio cholerae poses a significant public health threat, demanding improved diagnostic tools.
Purpose of the Study:
- * To develop a highly sensitive DNA-based electrochemical biosensor for Vibrio cholerae detection.
- * To utilize gold nanocubes and a modified glassy carbon electrode for enhanced sensing capabilities.
- * To validate the biosensor's performance in terms of sensitivity, selectivity, and repeatability.
Main Methods:
- * Fabrication of a glassy carbon electrode modified with gold nanocubes and APTES.
- * Electrochemical characterization using Electrochemical Impedance Spectroscopy (EIS) and Cyclic Voltammetry (CV).
- * Validation of sensor performance through DNA detection assays and analysis of bacterial cultures and real samples.
Main Results:
- * The biosensor demonstrated exceptional sensitivity with a wide linear dynamic range for target DNA detection.
- * A low limit of detection (LOD) of 7.41 × 10-30 molL-1 was achieved.
- * Selective detection of Vibrio cholerae was confirmed, with good repeatability over six assays and excellent recovery in poultry feces samples.
Conclusions:
- * The developed DNA biosensor offers a powerful and sensitive platform for detecting Vibrio cholerae.
- * The sensor's high sensitivity, selectivity, and stability make it suitable for various applications.
- * This technology holds promise for advancing pathogenic microorganism screening in clinical diagnostics, food safety, and environmental monitoring.
