Soil arsenic toxicity differentially impacts C3 (barley) and C4 (maize) crops under future climate atmospheric CO₂

Soil arsenic (As) contamination limits global agricultural productivity. Anthropogenic emissions are causing atmospheric CO₂ levels to rise. Elevated CO₂ (eCO₂) boosts plant growth both under optimal and suboptimal growth conditions. However, the crop-specific interaction between eCO₂ and soil arsenic exposure has not been investigated at the whole plant, physiological and biochemical level. Here, we tested the effects of eCO₂ (620 ppm) and soil As exposure (mild and severe treatments, 25 and 100 mg As/Kg soil) on growth, photosynthesis and redox homeostasis in barley (C3) and maize (C4). Compared to maize, barley was more susceptible to soil As exposure at ambient CO₂ levels. Barley plants accumulated more As, particularly in roots. As accumulation inhibited plant growth and induced oxidative damage in a species-specific manner. As-exposed barley experienced severe oxidative stress as illustrated by high H₂O₂ and protein oxidation levels. Interestingly, eCO₂ differentially mitigated As-induced stress in barley and maize. In barley, eCO₂ exposure reduced photorespiration, H₂O₂ production, and lipid/protein oxidation. In maize eCO₂ exposure led to an upregulation of the ascorbate-glutathione (ASC/GSH)-mediated antioxidative defense system. Combined, this work highlights how ambient and future eCO₂ levels differentially affect the growth, physiology and biochemistry of barley and maize crops exposed to soil As pollution.