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Microbiota of the Large Intestine

Microbiota of the Large Intestine

The large intestine hosts the most densely populated microbial ecosystem in the human body. This complex community primarily consists of anaerobic bacteria, with Bacillota (formerly Firmicutes) and Bacteroidota (formerly Bacteroidetes) as the predominant groups. The distribution of these microbes varies along different sections of the large intestine, influenced by local environmental factors such as oxygen availability and nutrient composition.The cecum, located at the beginning of the large...

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Related Experiment Video

Updated: Jun 30, 2026

Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts

Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts

Published on: April 29, 2014

Mapping the colorectal tumor microbiota.

C L Murphy^1,2, M Barrett^1,3, P Pellanda^1,3

¹APC Microbiome Ireland, University College Cork, National University of Ireland, Cork, Ireland.

|May 25, 2021

Summary

This summary is machine-generated.

The gut microbiome within a colorectal cancer (CRC) tumor is spatially homogeneous. A single biopsy is sufficient for accurately profiling the CRC tumor microbiome, reducing sampling error concerns.

Keywords:

Colorectal cancer gut microbiome tumor

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Last Updated: Jun 30, 2026

Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts

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Visualization of Gut Microbiota-host Interactions via Fluorescence In Situ Hybridization, Lectin Staining, and Imaging

Visualization of Gut Microbiota-host Interactions via Fluorescence In Situ Hybridization, Lectin Staining, and Imaging

Published on: July 9, 2021

Area of Science:

Gastroenterology
Microbiology
Oncology

Background:

The gut microbiome composition differs in colorectal cancer (CRC) patients compared to healthy individuals.
Previous research on the CRC tumor microbiome relied on single biopsies, raising concerns about sampling errors due to intra-tumor heterogeneity.

Purpose of the Study:

To investigate the spatial homogeneity of the gut microbiome within individual colorectal cancer (CRC) tumors.
To determine if microbiome composition varies significantly across different sites within a single tumor.

Main Methods:

Collected seven biopsies from various locations (on and off the tumor) from five CRC patients.
Analyzed and compared the microbiome composition and alpha-diversity across all samples from each individual patient.

Main Results:

Microbiome composition was highly similar across all biopsies from the same individual patient.
Microbiome alpha-diversity varied significantly more between different patients than within samples from a single patient.
Demonstrated spatial homogeneity of the gut microbiome within individual CRC tumors.

Conclusions:

The gut microbiome within a colorectal cancer (CRC) tumor is spatially homogeneous.
A single tumor biopsy is representative of the entire tumor's microbiome.
Microbiota alterations in CRC are not confined to specific tumor regions, supporting the use of single biopsies for microbiome profiling.