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Related Experiment Video

Updated: Nov 4, 2025

An In-vitro Preparation of Isolated Enteric Neurons and Glia from the Myenteric Plexus of the Adult Mouse
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Mouse Enteric Neuronal Cell Culture.

Yonggang Zhang1, Wenhui Hu2

  • 1Center for Stem Cell Research and Application, Institute of Blood Transfusion, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), Chengdu, China.

Methods in Molecular Biology (Clifton, N.J.)
|May 25, 2021
PubMed
Summary
This summary is machine-generated.

Researchers developed two methods to culture enteric neurons from mouse guts. These techniques provide a valuable model for studying gastrointestinal functions and related disorders.

Keywords:
Cell CultureEnteric NeuronsMouseNeural Stem/Progenitor CellsNeuronal Differentiation

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Area of Science:

  • Neuroscience
  • Gastroenterology
  • Cell Biology

Background:

  • The enteric nervous system (ENS) comprises numerous intrinsic neurons controlling gastrointestinal functions.
  • Culturing enteric neurons is crucial for physiological, electrophysiological, and pharmacological research.

Purpose of the Study:

  • To describe two effective methods for obtaining sufficient enteric neurons.
  • To provide a model system for studying ENS functions.

Main Methods:

  • Directly culturing primary neurons isolated from mouse myenteric plexuses.
  • Inducing neuronal differentiation of enteric neural stem/progenitor cells from mouse myenteric plexuses.

Main Results:

  • Both methods yield sufficient numbers of enteric neurons for experimental use.
  • The cultured neurons serve as a viable model for ENS research.

Conclusions:

  • Established methods allow for the successful culture of mouse enteric neurons.
  • These neuronal cultures are essential for advancing research in neurogastroenterology.