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Updated: Nov 4, 2025
Isolation of Primary Murine Brain Microvascular Endothelial Cells
Published on: November 14, 2014
Isolation of Primary Human and Rodent Brain Microvascular Endothelial Cells: Culturing, Characterization, and
Nune Darbinian1,2, Armine Darbinyan3, Nana Merabova4
1Department of Neurology, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, USA. nsarkiss@temple.edu.
Abstract:
Studies of blood-brain barrier (BBB) require developing of a novel and convenient in vitro endothelial cell model. We isolated primary human and rodent brain microvascular endothelial cells and developed methods for culturing, characterization, and high-efficiency transfection of endothelial cells. Here, we describe the improved methods to obtain in vitro human and rodent BBB models to study expression of endogenous and exogenous genes of interest.
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