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A Triple-Parameter-Based Laboratory-Friendly Fluorescence Imaging to Identify Apoptosis in Live Cells
Pradip De1,2, Jennifer Carlson Aske1, Nandini Dey3,4
1Translational Oncology Laboratory, Avera Cancer Institute, Sioux Falls, SD, USA.
Abstract:
Cellular signals to resist apoptosis have been attributed as one of the mechanisms of tumorigenesis. Hence, apoptosis is a cardinal target for drug development in cancers, and several antitumor drugs have been designed to induce apoptosis in tumor cells. Recently, venetoclax, a Bcl2 inhibitor that induces apoptosis, has been approved by the FDA for the treatment of CLL and SLL patients. Proapoptotic antitumor drugs have been traditionally developed and tested, targeting apoptosis in tumor cells. The mechanism of such drug actions has been functionally connected to the mechanism of apoptosis. The identification of apoptosis in a tumor cell takes into account different characteristics in several steps of apoptosis. Thus, it is understandable that modes of identification of apoptosis observed in tumor cells in a laboratory have also been tuned to different characteristics in several parameters of apoptosis. Here, we present a detailed methodology for a triple-parameter-based co-fluorescence imaging to identify apoptosis in live tumor cells. The procedure involves co-fluorescence staining specific for three cardinal features of apoptosis in live cells. The procedure is simple, time-sensitive, and can be performed successfully in a laboratory-friendly manner.
Insights
This study introduces a new, simple method using triple-parameter co-fluorescence imaging to identify apoptosis in live tumor cells. This technique aids in developing novel anti-cancer drugs targeting programmed cell death.
Area of Science:
- Biochemistry
- Cell Biology
- Oncology
Background:
- Apoptosis, or programmed cell death, is crucial in cancer development and a key target for anti-cancer drug discovery.
- Drugs like venetoclax, a Bcl2 inhibitor, are approved for treating certain cancers by inducing apoptosis.
- Current methods for identifying apoptosis in tumor cells can be complex and varied.
Purpose of the Study:
- To present a detailed methodology for identifying apoptosis in live tumor cells.
- To establish a reliable and accessible laboratory technique for apoptosis detection.
- To support the development of proapoptotic anti-cancer therapies.
Main Methods:
- Developed a triple-parameter-based co-fluorescence imaging technique.
- Utilized co-fluorescence staining targeting three cardinal features of apoptosis.
- Focused on identifying apoptosis in live tumor cells.
Main Results:
- The presented methodology allows for the identification of apoptosis in live tumor cells.
- The procedure is described as simple, time-sensitive, and laboratory-friendly.
- This technique enables the simultaneous assessment of multiple apoptosis characteristics.
Conclusions:
- A novel, efficient, and accessible method for detecting apoptosis in live tumor cells has been established.
- This triple-parameter co-fluorescence imaging technique can advance cancer research and drug development.
- The methodology facilitates a deeper understanding of apoptosis mechanisms in tumor cells.
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