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Updated: Nov 4, 2025
Author Spotlight: A Streamlined Approach to Studying Cell Death Initiation in Hypersensitive Response
Published on: November 10, 2023
Kinetic Measurement of Apoptosis and Immune Cell Killing Using Live-Cell Imaging and Analysis
Jill E Granger1, Daniel M Appledorn2
1Essen Biosciences/Sartorius, Ann Arbor, MI, USA.
Abstract:
The rapid, efficient detection of cell death is critical for characterizing the underlying biology of in vitro disease models and, in particular, immunotherapy products used for preclinical therapeutic research. Traditional endpoint assays are laborious to perform for mass screening of therapeutic candidates and may fail to fully capture the kinetics of events surrounding the initiation, duration, and mechanisms of cell death-important events that may affect translational relevance and impact therapeutic decision-making during development. Here, we describe simple, efficient methods to measure apoptosis and immune cell killing in both adherent and nonadherent cell populations using the Incucyte® Live-Cell Analysis system and associated nonperturbing reagents, cells, and protocols. Assays are performed in the user's own incubator with minimal disturbance and may be readily incorporated into existing workflows. Users may multiplex to maximize data collection from each sample. The integrated, user-friendly software does not require advanced technical training, enabling rapid analysis. Taken together, this method provides essential kinetic insight for greater understanding of cell death and the dynamic interactions between immune cells and their targets.
Insights
This study introduces a simple, live-cell analysis method for measuring apoptosis and immune cell killing in preclinical research. The Incucyte system offers efficient kinetic insights into cell death, improving therapeutic development.
Area of Science:
- Immunology
- Cell Biology
- Preclinical Research
Background:
- Accurate cell death detection is crucial for in vitro disease models and immunotherapy research.
- Traditional assays are time-consuming and may not capture the full kinetics of cell death events.
- Understanding cell death dynamics is vital for therapeutic decision-making.
Purpose of the Study:
- To describe simple and efficient methods for measuring apoptosis and immune cell killing.
- To utilize the Incucyte Live-Cell Analysis system for real-time cell death assessment.
- To provide kinetic insights into cell death and immune cell interactions.
Main Methods:
- Employed the Incucyte Live-Cell Analysis system with nonperturbing reagents.
- Developed assays for both adherent and nonadherent cell populations.
- Integrated multiplexing capabilities for enhanced data collection.
Main Results:
- Demonstrated simple and efficient measurement of apoptosis and immune cell killing.
- Enabled assays within the user's incubator with minimal disturbance.
- Provided user-friendly software for rapid data analysis without advanced training.
Conclusions:
- The described method offers essential kinetic insight into cell death.
- Facilitates a greater understanding of dynamic interactions between immune cells and targets.
- Supports improved preclinical therapeutic research and development through efficient cell death analysis.
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