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Related Experiment Videos

Adenosine transport by the lung.

D K Das1, H Steinberg

  • 1Cardiovascular Division, University of Connecticut School of Medicine, Farmington 06032.

Journal of Applied Physiology (Bethesda, Md. : 1985)
|July 1, 1988
PubMed
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The lung efficiently removes adenosine from circulation, utilizing it for adenine nucleotide synthesis. This uptake is a saturable, passive process mediated by a specific, rate-limiting transport system.

Area of Science:

  • Pharmacology
  • Pulmonary Physiology
  • Nucleoside Transport

Background:

  • Adenosine is a nucleoside with vasodilatory properties.
  • The lung is known to metabolize various circulating compounds.

Purpose of the Study:

  • To investigate the mechanism and characteristics of adenosine uptake by the lung.
  • To determine if adenosine transport is a rate-limiting step in its pulmonary metabolism.

Main Methods:

  • Isolated perfused rat lung model.
  • Infusion of radiolabeled [14C]adenosine.
  • Pharmacological inhibition of adenosine deamination and kinase activity.
  • Kinetic analysis of adenosine uptake (Km, Vmax).

Main Results:

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  • The lung removed 69.3% of infused adenosine in a single circulation.
  • Adenosine uptake was independent of its deamination.
  • Adenosine was transported into lung cells for adenine nucleotide synthesis.
  • Uptake was saturable, passive, and competitively inhibited by adenosine analogues.
  • Kinetic parameters (Km=0.227 mM, Vmax=4.6 mumol.min-1.g lung-1) indicated a specific transport system.

Conclusions:

  • A specific, saturable, and rate-limiting transport system facilitates adenosine uptake by the lung.
  • This transport is crucial for the intracellular utilization of adenosine, particularly for adenine nucleotide synthesis.